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KMID : 0364820130490020137
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Korean Journal of Microbiology
2013 Volume.49 No. 2 p.137 ~ p.145
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Changes of Nitrifying Bacteria Depending on the Presence and Absence of Organic Pollutant in Nak-Dong River
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Jin Seon-Yeong
Lee Young-Ok
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Abstract
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This study was performed at 2 sites of Nak-Dong River to investigate the changes of nitrifiers depending on the
presence and absence of organic pollutants (due to the effluents of domestic wastewater treatment plant, WWTP).
Conventional chemical parameters such as T-N, NH4-N, NO2-N, NO3-N were measured and the quantitative nitrifiers
at the 2 sites were analyzed comparatively by fluorescent in situ hybridization (FISH) with NSO190 and NIT3, after checking the presence of gene amoA of ammonia oxidizing bacteria (AOB) and 16S rDNA signature sequence for Nitrobacter sp. that belongs to nitrite oxidizing bacteria (NOB). Also α·β·γ-Proteobacteria were detected using FISH to get a glimpse of the general bacterial community structure of the sites. Based on the distribution structure of the α·
β·γ-Proteobacteria and the measurement of nitrogen in different phases, it could be said that the site 2 was more polluted with organics than site 1.
Corresponding to the above conclusion, the average numbers of AOB and NOB detected by NSO160 and NIT3,
respectively, at site 2 [AOB, 9.3×105 ; NOB, 1.6×106 (cells/ml)] was more than those at site 1 [AOB, 7.8×105 ; NOB,
0.8×106 (cells/ml)] and also their ratios to total counts were higher at site 2 (AOB, 27%; NOB, 34%) than those at site 1 (AOB, 18%; NOB, 23%). Thus, it could be concluded that the nitrification at site 2 was more active due to continuous loading of organics from the effluents of domestic WWTP, compared to site 1 located closed to raw drinking water supply and subsequently less polluted with organics.
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KEYWORD
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FISH, nitrifier, organic pollutant, PCR-DGGE
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